Correlation of Biochemical Parameters in Viral Hepatitis Patients.

Abstract

Objective: Observation of biochemical parameters in the subjects with viral hepatitis in relation to their cause and stage of disease.

Design: Observational study.

Place & Duration:  The  research  was performed at the Department of Biochemistry in Basic Medical Sciences Institute (B M.S.I), situated at the J.P.M.C. Karachi, for the period of twelve months.

Material  & Methods: The patients diagnosed with viral hepatitis B or hepatitis C infection were incorporated in current research. Selection of subjects was done after diagnosis by ELISA method of their disease. Controls  for the study were selected  those  subjects who were negative for Hepatitis B and Hepatitis C. Serum insulin levels were estimated by ELISA method. While blood glucose by Hexokinase method  and prothrombin  time (PT) was estimated by one stage coagulation technique. However liver enzymes  were assayed by enzymatic method. For paired and correlation data analysis, all comparisons, up to 0.05 P value was considered significant.

Result: The mean values of fasting blood glucose, insulin and albumin as compared to control were statistically  significant (P<0.05). In all groups, the mean values of AST and  ALT  were found statistically highly significant (P<0.01), which indicates liver damage progression with subsequent rise in serum levels of insulin in these subjects.

Conclusion: The patients diagnosed positive  either  for  viral hepatitis B and C, should also be screened for insulin in addition to hepatic profile to decrease fatal outcome of the co-morbid condition.