PROTECTIVE ROLE OF ZINC CHLORIDE AGAINST TOXICITY OF CIPROFLOXACIN ON FOETAL HEPATOCYTES OF ADULT WISTAR ALBINO RATS.

Abstract

Objective: This investigation was aimed to evaluate the unknown defensive function of zincin mitigating the toxicity levelstimulated by ciprofloxacin in the liver tissues of Wistar albino rats. This research was carried out as a prospective study. Setting/Design: The study has been done in the Department of Anatomy, Pir Abdul Qadir Shah Jeelani Institute of Medical Sciences Gambat, and District Khairpur Mir’s. Pakistan. Time Duration: One year of studyhas been completed from March 2019 to February 2020. Method: Separate doses of Ciprofloxacin and Zinc chloride has been given to female Wistar albino rats during their gestation period. Different concentrations of 20 mg/kg of body weight and 1200 g/kg of body weight have been used. Doses of Ciprofloxacin and Zinc chloride have been injected intraperitoneally two times daily. Doses have been given from 8th day of gestation to 18th day of gestation. Albino rats were divided into three groups A, B and C. Each group contains 40 specimens; all the selected animals were treated by deep ether anesthesia and then dissected to remove the attached fetuses from the body of the females. Sexes of those fetuses were not considered. All the fetuses were dissected on the 18th day of gestation to get their liver. Weighing and fixation of liver were done by using 10% non-buffered formalin, Implantation has been done by using paraplast and thick sections of about 3 micrometers were cut by using rotary microtomy techniques. Staining of microtomic sections was done with the help of hematoxylin and eosin stains. The morphology histopathology of the liver tissues has been observed. Their structural differences have been compared with the histological patterns of liver tissues of controlled animals. The data has been compiled and evaluated statistically. Result:This study resulted the adverse effects of ciprofloxacin on the liver tissues as it was found that weight of liver from treated animals were remarkably decreased as equated to control animals. Histological evaluation has also been done.Hepatocytes was found effected by the doses of ciprofloxacin as decrease in sizes and number has been noted. Sizes of nuclei were also observed lesser as compared to controlled samples. The use of Zinc chloride along with ciprofloxacin has been shown to control the modified state of histological properties into the average conditions. Conclusion: Progressive changes have been observed in morphological conditions of hepatocytes of