EFFECT OF OMEGA-3 AND 6 POLYUNSATURATED FATTY ACIDS ON DIABETES MELLITUS TYPE 2.
Keywords:Omega-3, 6, PUFA polyunsaturated fatty acids, insulin markers, Alloxan, Albino rats.
OBJECTIVES: To evaluate the effects of optimized proportions of Omega-3 and Omega-6
polyunsaturated fatty acids on diabetes mellitus type 2.METHODOLOGY: The experimental analytical
study was carried out in the research lab of Isra University Hospital Hyderabad from March to August 2022.
A total 50 male healthy albino Wistar rats weighing about 200 250 grams were procured from the animal
house of Sindh Agricultural University Tandojam. After completion of the acclimatization period, rats were
initially divided into group A and B Control n=10 and Experimental groups n=40 respectively. The control
group received a standard chow diet and water ad libitum for one month. The experimental group rats were
injected with Alloxan 150mg/kg body weight mixed with 2ml normal saline intra-peritoneally to induce
diabetes in the experimental groups. After induction experimental rats were further divided into 4 groups
n=10. Group B1 Diabetic control group after inducing diabetes fed with a normal chow diet ad labitum for
30 days. Group B2 Experimental Omega-3 Treated Group was fed a diet mixed with 0.3gms/kg b.w of
Omega-3 fatty acid for 30 days.Group B3 Experimental Omega-6 Treated Group fed diet mixed with
0.3gms/kg b.w of Omega-6 fatty acid for 30 days. Group B4 Experimental Combination Treated Groups
was fed a diet mixed with equal amounts of Omega-3 and Omega-6 fatty acids in a dose of 0.3gms/kg b.w
for 30 days.RESULT: A significant difference between the mean body weight of rats of groups A, B1, B2,
B3, B4 p<0.05. The mean level of FBS before and Mean level of FBS after induction showed statistically
significant at p-values <0.05 and <0.001 respectively. The mean level of C-reactive proteinmg/L in Control
was 0.11±0.03 while in group B1 rats was 0.61±0.12 in B2 rats was 0.24±0.04, in B3 rats was 0.31±0.08
and in B4 rats was 0.17±0.03. The C reactive levels were found to be significantly elevated in group B1
compared with A, B2, B3, and B4 rats. CONCLUSION: It is concluded from the present study that the
administration of equal proportions of Omega-3 and Omega-6 polyunsaturated fatty acids plays a
significant role in regulating body homeostasis and also impacts glycemic control by improving markers of
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