INVESTIGATING THE ANTIOXIDANT EFFECT OF VITAMIN E AGAINST MSG INDUCED OXIDATIVE STRESS ON OVARIES OF WISTAR ALBINO RATS.

JPUMHS; 2025:15:01,64-70. http://doi.org/10.46536/jpumhs/2025/15.01.597

Abstract

BACKGROUND: MSG, a flavoring agent is well known for its toxic effects on reproductive
system due to its oxidative stress which may leads to infertility. Antioxidants like Vit E helps
to overcome this issue. OBJECTIVE: To evaluate the toxic effects of MSG on the ovaries of
Albino Wistar rats and the possible protective effects of vitamin E on damaged ovarian tissues.
STUDY DESIGN: Experimental study. PLACE OF STUDY: The study was performed at
the Animal House department, Sindh Agriculture University Tandojam and, the department
of Anatomy and Postgraduate laboratory of Isra University Hyderabad. DURATION OF
STUDY: 6 months. MATERIALS AND METHODS: 40 Albino Wistar rats were divided
into 4 groups. Among them first was controlled group, second was treated with 0.08mg/g of
MSG daily, third and fourth were treated with Vit E in the dosage of 0.4mg/g for 30 and 15
days respectively. After a month the animals were sacrificed and slides of 4-6 µm of thickness
were made for examination. Spss version 21. and MS Excel was used, chi-square test was
applied at the P value of <0.05. RESULTS: The results show that Group A shows no sign of
damage, Group B shows hemorrhage, atretic follicles, vacuolation, distorted basement
membrane, hypertrophied theca cells, and less mature follicles while Group C shows a marked
reduction in all of this damage whereas Group D shows improved results on all of these toxic
effects other than vacuolation and atretic follicles. CONCLUSION: MSG causes damage to
ovarian tissue and contrary to this Vit E shows a protective effect against this damage.