Vitamin D3 & Serum Calcium Levels in Premenstrual Syndrome

Abstract

Objective To determine vitamin D3 and serum calcium levels in premenstrual syndrome during luteal phase. Study Design : Comparative case control study Place and Duration: Department of Gynecology and Obstetrics and Department of Biochemistry, Isra University Hospital from June-December 2013. Material & Methods: A sample of 85 unmarried female of age 18-28 years were selected through non-probability purposive sampling according to pre-defined inclusion and exclusion criteria. Subjects were divided in; to Group I, normal healthy female as controls (n=42) and Group H diagnosed cases of premenstrual syndrome (n=43). Serum 25-0H-D3 was estimated by ARCHITECT I 1000 system and serum calcium by Roche Automated Clinical Chemistry Analyzer 902 modular system. Data was analyzed on SPSS version 21.0. The continuous and categorical variables were analyzed by student's t-test and chi-square test respectively. Pearson's correlation was used to determine association between vitamin D3 and serum calcium. The significant p-value was taken at = 0.05. Results : Mean±S.D age of controls and premenstrual syndrome (PMS) cases noted was 22.6±3.2 and 21.6±3.9 years (range 18-28 years) (p=0.91). The premenstrual subjects revealed a significantly low vitamin D3 levels compared to controls. The mean±S.D of vitamin D3 was noted as 26.20±4.30 and 14.88±2.39 ng/dI respectively. (13=0.0001). Vitamin D3 levels as low as 8 ng/d1 were noted in premenstrual syndrome. Significant statistical difference was observed for serum calcium level between groups i.e., 9.30±0.81 vs. 8.52±1.18 (p=0.001) Positive correlation co-efficiennt was observed between serum Vitamin D3 and calcium levels (r=0.44, p=0.001). Conclusion: The present study observed low vitamin D3 and calcium levels in female subjects suffering from premenstrual syndrome. Supplementation with vitamin D3 and calcium may help to alleviate the symptoms of premenstrual syndrome.