Comparison of Cardiac Eeffects of Avicennia Marina Digoxin on Isolated Rabbit's Heart

Abstract

Objective: To compare the cardiac effects of aqueous extract of Avicennia marina and digoxin on the isolated rabbit's heart by Langendorffmethod. Study Design: Experimental interventional study. Place & Duration: Experimental work was completed in five months duration at BMSI, JPMC, Karachi. Material & Methods: An aqueous extract obtained from the leaves of traditional medicinal plant Avicennia marina (Mangrove plant) tested on isolated heart of Rabbit and its effect were compared with digoxin, using the langendorff heart preparation. Administration of extract and digoxin into the retrograde perfusion solution induced cardio-activity in vitro and their effect compared in presence ofAtenolol and Verapamil. Results: Aqueous extract of Mangrove plant leaves possessed positive inotropic and negative chronotropic effect in langendorff method of heart preparation. Similar effect is also produced by digoxin as mentioned in standard medical text books. Pre-treatment with Atenolol and Verapamil separately failed to modify the cardiac effect produce by aqueous extract of Avicennia marina and digoxin. Our observations showed similarity in responses of extract and digoxin. The effect produced by aqueous extract and digoxin in presence of Atenolol and Veraparriil respectively have similarity in cardiac effect. Conclusion: Mangrove plant extract showed increased in force of contraction and decreases in rate of isolated mammalian heart, as a similar effect produced by digoxin, and which shows the similarity in their action even in presence ofAtenolol and Verapamil respectively.