Restoration of Stress - Related Apoptosis and Altered Splenic Immunoarchitecture with Cyanocobalamin

Abstract

Objective: To evaluate role of the Cyanocobalamin on the deleterious effects of heat stress on the immunity and immune organs. Methods: This experimental study was conducted in the department of Anatomy, Basic Medical Sciences Institute, Jinnah Postgraduate Medical Centre, Karachi from January 2013 to April 2013, adult, healthy 45 male and female Sprague-Dawley rats of (180-200 g) were obtained from Animal house ofBMS1, JPMC Karachi, randomly divided in 3 groups of 15 rats in each. They further subdivided into 3 subgroups on the basis of treatment duration. Group A animals served as control. Group B animals received Heat-stress. Group C animals received Heat-stress + Cyanocobalmin. The animals were sacrificed, immune organ spleen dissected and fixed in alcoholic formal in for 24 hours. Then processed in ascending strengths of alcohol, cleared in xylene, infiltrated and embedded in paraffin. Five microns thick sections were cut on microtome, and stained with Haemotoxylin and eosin for detailed microscopy and micrometry with stage micrometer and ocular reticule. Results: Study reveals decreased B and T- cell count, marked reduction in size of all compartments of splenic nodules with characteristic appearance of tingible body macrophages in group B animals received heat stress. Whereas animals of group C who received protection with Cyanocobalamin in addition to heat-stress shows an insignificant decrease in B and T cell count, with few macrophages and prominent mitotic figures. Conclusion: It is concluded that Cyanocobalamin has expressed itself as an anti-heat stressor and an immunopotentiating agent under heat stress conditions for the individuals who work in wanner environment.