QUANTITATIVE MORPHOMETRIC ANALYSIS OF UTERINE SPIRAL ARTERY IN NORMAL AND PREECLAMPTIC PLACENTAE VIA FLUORESCENCE MICROSCOPY

Abstract

Back ground: Preeclampsia is a syndrome restricted to pregnancy. It affects approximately 10% of pregnancies throughout the world. Although a definite cause of preeclampsia has not been identified, compelling evidence implicates that factors indirectly affecting implantation of the placenta are involved in the etiopathogenesis. Objective: The aim of this study was to determine the association of abnormal remodeling of spiral arteries in pregnancy induced hypertension by comparing the parameters of uterine spiral arteries with the control group. Design: Case-Control Place of Study: The histological analysis was done at MDRL-2, Ziauddin University. Duration of study: April 2019- June 2020 Methodology: We took 21 normal placentae and 21 diagnosed preeclamptic placentae from Ziauddin Hospital, Kemari Campus. Histological analysis was done following standard protocols. On histological examination, the changes in lumen diameter and area, wall area and wallthickness via fluorescence microscopy were measured between the two groupsby using Nikon NIS-elements D software. Wall / lumen area ratio was also calculated accordinglybetween the two groups. Results: Our results demonstrated a statistically significant reduction in the lumen diameter and lumen area of the spiral arteries among the PIH group as compared to the control. We have also observed statistically significant increased wall thickness and wall area of the spiral arteries among PIH group as compared to the control. Conclusion: The results of our study suggest a significant alteration in the normal remodeling of spiral arteries due to decrease in the luminal parameters, and increased in wall thickness parameters of spiral artery which could be the reason for the development of pregnancy induced hypertension.