ESTIMATION OF CELL COUNT IN PARS DISTALIS OF PITUITARY GLAND AFTER PRENATAL ETHANOL EXPOSURE IN THE RAT.
Keywords:Rats, Ethanol, Pituitary gland, Cell count, Growth
OBJECTIVE: To investigate the effects of prenatal exposure of ethanol on the total number of cells in
pars disatlais of pituitary gland of rat pups raised to adults.
STUDY DESIGN: Experimental study.PLACE AND DURATION OF STUDY: Department of
anatomy, College of Physician and Surgeons, Regional Centre, Islamabad, between April 2014 and April
- METHODOLOGY: This experimental study was done on 16 female Sprague Dawley rats, which
were selected from NIH using random sampling method. Female rats were mated with four male rats and
their pregnancy was confirmed by presence of spermatozoa in their vagina. The female rats were then split
into two groups, experimental group ‘B’ and control group ‘A’, and used for reproduction. Group A
received injections of normal saline from the 10th to the 18th gestational day GD while Group B received an
intraperitoneal ethanol injections at a dose of 4.44ml/kg. Until their normal gestation and delivery at GD
21 to 23, animals were maintained in conventional laboratory settings. Pups that were males were raised
until their 70th day. Periodic acid Schiff Orange G PAS-OG was used to stain pituitary glands, which were
then used for histological analysis. RESULTS: As compared to the control group’s mean acidophil count
46.93± 0.71, p < 0.000, the experimental group mean acidophil count revealed a reduction 20.81 ± 0.90.
Additionally, there was a decrease in the mean basophil count in the experimental group 5.46 ± 0.78 vs
10.89± 0.96, p < 0.000. When compared to the control group, the experimental groups mean chromophobe
count increased 57.82 ± 0.96 vs 46.46± 1.09, p < 0.000.CONCLUSION: Comparing the exposed group to
the non-exposed group, there was a decrease in chromophil count and an increase in chromophobe count.
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